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Dr Kan Mun Seng

Lecturer

PhD ( University of Sheffield, UK)

Telephone: +603 - 5514 6118
Fax: +603 - 5514 6364
E-mail: kan.mun.seng@artsci.monash.edu.my

Links
Area of Expertise
Research Interest
Selected Journals
Current Research Projects
Prospective Research Projects
Research Students

Area of Expertise:

Molecular gene cloning, protein expression and purification, enzyme technology, bio-fermentation

Research Interest:
  • Gene cloning and expression of carbohydrate degradation enzymes.
  • Bioethanol production using various feedstocks in fermentation.
  • Extraction of immunopotentiators from macro fungi .
Selected Journal Papers:

Pinarbasi E, Kan MS, Duran C, Ford GC, Hornby DP (1998) Substitution of the conserved phenylalanine in the S- adenosyl-L-methionine binding site of M.MspI with tyrosine modifies the kinetic properties of the enzyme. Biol Chem 379: 591-594

Current Research Projects:
  • Extraction of immunopotentiators from Lentinula edodes (shiitake cultivar flower mushroom) and Pleurotus ostreatus (oyster mushroom).
  • Cloning and expression of xylanase from Bacillus subtilis.
Prospective Research Projects :

Gene cloning

  • Cloning and overexpression of xylose isomerase gene
  • Subcloning and mutagenesis study of pullulanase gene
  • cDNA cloning of Lignin peroxidase from local white-rot fungi isolates

Fermentation

  • Optimization of bio-ethanol fermentation as biofuel
  • Optimization of polyhydroxy alkanoate (PHA) fermentation as bio-plastic
Bioactive compound
  • Extraction of immunopotentiators from local wild macro fungi isolates
Research Students :

Honours students ( Present )

  • Ms Asiri Perera (2008) Qualitative and quantitative studies on cassava starch fermentation leading to biofuel production.
  • Ms Ling Suk Juin (2008) Expression of recombinant Pullulanase gene from Bacillus subtilis in Escherichia coli.

 

Honours students (Past)

  • Ms Tan Wei Lin (2007) Determination of the optimum conditions for polyhydroxyalkanoate (PHA) biosynthesis form Pseudomonas aeruginosa.
  • Ms Lim Wei Ling (2007) Molecular determination of lignin peroxidase lip gene from Trametes menziezii and Aspergillus isolates.
  • Ms Irene Lee Mei Yean (2005) Molecular cloning and expression of pullulanase amyX gene from Bacillus subtilils.
  • Mr Sandun Dalpadatu (2005) Determination of optimum conditions for alcohol production from starch by mixed cultures.
  • Ms Goh Yuh Shan (2003) Isolation and cloning of the polyhydroxyalkanoate (PHA) biosynthesis genes from two Pseudomonas isolates.
  • Ms Liam Lian Chien (2003) C loning and expression of amylase gene amyE from Bacillus subtilis .